Kimiko Date-Krumm has lost her Australian Open third-round match in straight sets to Bojana Jovanovski.
The 42-year-old, who became the oldest woman ever to win a match in the tournament earlier this week, fell 2-6 6-7 to the Serbian.
The Japanese player had a conservative approach to the match, making 18 fewer unforced errors than her opponent, but also managing to hit just nine winners.
The 21-year-old's more aggressive approach paid off, giving her seven break points against the Date-Krumm serve throughout the match.
Jovanovski, who is unseeded for the tournament, will face Sloane Stephens in the fourth round.