Laura Robson has suffered defeat against Ellie Halbauer at the second-round stage of a Challenger event in Canada.
The Briton picked up a first singles victory in nearly two years on Wednesday, but she was unable to overcome Halbauer in her latest outing.
Robson, now ranked at 913 in the world following a lengthy injury layoff, fell 6-2 4-6 6-2 in a time of two hours and 31 minutes.
Despite the setback, the 21-year-old will still compete at next month's US Open as a result of her protected ranking.