Gerard Pique pulled up with a groin injury in Barcelona's 1-1 draw against Eintracht Frankfurt in the first leg of their Europa League quarter-final on Thursday night.
The 35-year-old picked up the muscle problem after slipping while attempting to make an interception, and when the centre-back realised he was unable to continue, he was replaced by Clement Lenglet in the 22nd minute.
Even though Pique is in the latter years of his career, the Spain international is still regarded as an important player for Xavi's side, and he has played in the vast majority of Barcelona's matches across all competitions this season, featuring in 38 games and missing only four due to injury.
The veteran defender, however, is now set for a spell on the sidelines, with the Catalan giants confirming on their official website that "further tests will be required" to determine the severity of his injury.
Pique is unlikely to feature for Barcelona in Sunday's La Liga encounter with Levante and he will also be a major doubt for their Europa League second-leg clash with Frankfurt at Camp Nou next Thursday.
After going behind in the 48th minute at the Deutsche Bank Park, a 66th-minute strike from Ferran Torres restored parity for the visitors, which leaves the quarter-final tie nicely poised heading into next week's second-leg battle in Catalonia.
No Data Analysis info
Germany
Switzerland
Scotland
Hungary
Spain
Italy
Albania
Croatia
England
Denmark
Slovenia
Serbia
Netherlands
France
Austria
Poland
Romania
Belgium
Slovakia
Ukraine
Portugal
Turkey
Czech Republic
Georgia