Serie A is the best-represented league in the last 16 of the Europa League with five teams present, but at least one of those is guaranteed to be eliminated as Roma face Fiorentina at the Stadio Olimpico.
The all-Italian tie is evenly poised after last week's 1-1 draw in the first leg at the Stadio Artemio Franchi.
Hosts Fiorentina took the lead in the 17th minute through Josip Ilicic, but it wouldn't be enough for victory as Seydou Keita equalised an hour later for a crucial Roma away goal.
It means that Rudi Garcia's side need a goalless draw or better at home to advance to the quarter-finals, but they do not enter the match in good form.
The Giallorossi have won just three of their last 15 games in all competitions and fallen behind Juventus in the Serie A title race following a five-game winless domestic run.
They have drawn most of their games of late, but on Monday saw their 16-game unbeaten league run, which stretched back to November 1 last year, ended at home by Sampdoria.
Garcia will be without the suspended Radja Nainggolan after he was booked in the first leg, while talismanic skipper Francesco Totti is a doubt.
La Viola, meanwhile, will be confident of grabbing an away goal as they have scored in 13 of their last 14 Europa League fixtures, while Roma have gone 21 matches in European competition without a clean sheet.
Vincenzo Montella's outfit are one of the sides on second-placed Roma's tail in Serie A and will enter Thursday's game on the back of a last-gasp win over AC Milan on Monday.
Former Roma player Montella will be without key forward duo Mario Gomez and Giuseppe Rossi, meaning that Ilicic should again lead the line.
If Fiorentina avoid defeat at the Stadio Olimpico, they will become the first side to go 10 successive away matches in the Europa League proper without losing.
This will be the fifth meeting between the two sides this season and Fiorentina will hope to emulate their last trip to the capital when they won 2-0 in the Coppa Italia quarter-finals last month.
Roma:
Form in Europa League: DWD
Form in all competitions: DWDDDL
Possible starting lineup: De Sanctis; Torosidis, Mapou, Astori, Cholevas; Pjanic, Keita, Florenzi; Gervinho, Totti, Iturbe
Fiorentina:
Form in Europa League: DWLDWD
Form in all competitions: WWWLDW
Possible starting lineup: Neto; Tomovic, Rodriguez, Basanta, Alonso; Badelj, Pizarro, Borja Valero; Salah, Ilicic, Joaquin
Sports Mole says: 1-1
Pascal Lemesre
