Leander Dendoncker also netted but Jota made it five goals in two games after his brace at Brighton on Sunday, with Nuno insisting he must not let his standards drop.

Nuno said: "He must do what he is doing every day, be really competitive, train well and prepare himself.

"Be like he is on the pitch, always fighting, always looking for space, always running. Keep on keeping going.

"When you make something (a substitution) you try to change the game and try to find the best solution, sometimes it works out sometimes it doesn't.

"We want players to make an impact, Diogo did fantastically, well done."

After a forgettable first half, Jota was thrown on after 56 minutes and by the 68th he had scored three times and Wolves were 4-0 up.

He tapped in Pedro Neto's cross with his first touch 77 seconds after coming on before netting after Dendoncker's drive was turned onto the bar by Utku Yuvakuran.

Dendoncker's header made it 3-0 and Jota completed his hat-trick a minute later, turning in Oskar Buur's centre.

He became the first Wolves player to score a European treble since Derek Dougan in 1971.

Nuno added: "It was a good game, we played well, we scored goals, defended well and were organised. It was a well played game.

"It was a tough competition and we've progressively got better. We fight game-by-game and it's taken us to the knockout stage.

"We are delighted to be there but now we put it aside and wait for the draw."

Besiktas, who had won their previous six games, finished bottom of Group K and boss Abdullah Avci now wants to focus on their title challenge with his side second in the Super Lig.

He said: "The Europa League has been a disappointment for us, now we are going to analyse the lessons. Now we're back to the league.

"We made some changes and tried to give some chances to young players but between 55 and 75 minutes we lost our shape and concentration.

"We have good young players and I believe they can develop themselves and help Turkish football. Now is not the time to analyse the situation, we'll do that in the winter break."