Millwall manager Kenny Jackett has named an unchanged side from the one that knocked Aston Villa out of the FA Cup on Tuesday.
It means that John Marquis, who headed in the winner, keeps his place alongside Andy Keogh in attack.
Barnsley manager Keith Hill makes three changes to his side, with Stephen Foster, John Stones and Jim O'Brien all in the squad. New signing Jason Scotland makes the bench.
Barnsley: Steele; Golbourne, Cranie, Foster, Kennedy; Stones, Etuhu, O'Brien, Perkins; Dagnall, Harewood
Millwall: Forde; Smith, Shittu, Osborne, Beevers; Henry, Abdou, Trotter, Feeney; Marquis, Keogh