However, Roofe's dismissal for a brutal boot to the face of Prague keeper Ondrej Kolar was a fatal set-back which was quickly compounded by Balogun, who picked up a second yellow as he handed Nicolae Stanciu a set-piece chance he expertly sweep home the second.

There was another flash-point just before the end as the Gers players seemed to accuse Slavia's Ondrej Kudela of an offensive comment.

But it is Jindrich Trpisovsky's men who now march into Friday's draw for the last eight after following up their last-16 triumph over Leicester with another British scalp – leaving Gerrard's recently-crowned Scottish champions with only the Scottish Cup to strive for this term.

A barrage of fireworks sparked by fans outside the ground lit up the Ibrox sky as the game kicked-off but it was Slavia who exploded forward 14 minutes in.

Slavia skipper Jan Boril's cross found Olayinka, who got ahead of Connor Goldson to head home. Allan McGregor was the hero in the Eden Arena with last week's wonder save but this time the veteran keeper will be disappointed at allowing the ball to squirm through his grasp.

Gers had a title party hangover to blame for their slow start in Prague but this time there was no excuse other than the slickness of Slavia's build-up play.

Ryan Kent did bring a good save out of Kolar before Alfredo Morelos headed a golden chance over.

Little was going right for Gerrard's team. Israeli referee Orel Grinfeeld was taking a lenient approach with Prague's repeated fouls but chose to show Glen Kamara a yellow which rules him out of Gers' first Champions League qualifier next season for merely standing his ground while bouncing into Alexander Bah.

Balogun – starting ahead of last week's goalscorer Filip Helander – survived a VAR check just before the break as he scrambled back to recover his own mistake after gifting the ball to Abdallah Sima.

It was clear Gerrard needed to change something. He gave it 10 minutes into the second period before introducing Roofe for Scott Arfield.

But the former Leeds striker was only on for seven minutes before he was heading off again as he saw red for his shocking challenge on Kolar.

Chasing a long Goldson pass, Roofe lunged forward with a high boot that flew into the keeper's face – leaving blood gushing from a huge head wound.

As Roofe made his way for the tunnel, Kolar was stretchered off to be replaced by 18-year-old debutant Matyas Vagner.

But Rangers did not get a chance to test the youngster's nerve as Balogun followed up his booking for a first-half foul on Lukas Provod with another after a crunching tackle into Boril, which left Grinfeeld no option to flash red again.

And Rangers' remarkable continental run was finally killed off as Stanciu swept home a stunning free-kick.

A bad tempered affair threatened to boil over just before the end when Kudela whispered something in Kamara's ear, drawing a furious reaction from the Rangers players before the officials could restore calm.