Emyr Huws will play no part in Wales' Euro 2016 qualifier against Bosnia-Herzegovina on Friday due to an ankle injury.
The Wigan Athletic midfielder was already a doubt due to his fitness, but manager Chris Coleman has now confirmed that the 21-year-old will undergo a scan.
Wales, who lost Aaron Ramsey and Joe Allen to injuries prior to the squad announcement, is now without 10 players for the qualifiers.
After taking on Bosnia, Wales will play Cyprus on Monday.
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