Arsenal defender Calum Chambers and West Bromwich Albion striker Saido Berahino are among the 24-man party involved in the England Under-21s' upcoming fixtures against Czech Republic and Germany.
Manager Gareth Southgate has picked the duo, as well as Everton defender John Stones, after they were left out of Roy Hodgson's senior squad for the international break.
Southampton left-back Matt Targett has received his first call-up to the Under-21s due to his impressive performances in the Premier League this season, while his teammate James Ward-Prowse has returned to the squad.
There is also a place for Manchester United's Jesse Lingard, who has not featured for the Young Lions since May due to injury.
The Under-21s will face Czech Republic in Prague on March 27 and Germany in Middlesbrough three days later.
England Under-21 squad in full:
Goalkeepers: Marcus Bettinelli (Fulham), Jonathan Bond (Watford), Jack Butland (Stoke City)
Defenders: Calum Chambers (Arsenal), Eric Dier (Tottenham Hotspur), Luke Garbutt (Everton), Ben Gibson (Middlesbrough), Carl Jenkinson (West Ham United - loan from Arsenal), Michael Keane (Burnley), Liam Moore (Brentford – loan from Leicester City), John Stones (Everton), Matt Targett (Southampton)
Midfielders: Tom Carroll (Swansea City - loan from Tottenham Hotspur), Nathaniel Chalobah (Reading - loan from Chelsea), Jake Forster-Caskey (Brighton & Hove Albion), Will Hughes (Derby County), Jesse Lingard (Derby County - loan from Manchester United), Alex Pritchard (Brentford - loan from Tottenham Hotspur), Nathan Redmond (Norwich City), James Ward-Prowse (Southampton)
Forwards: Patrick Bamford (Middlesbrough - loan from Chelsea), Saido Berahino (West Bromwich Albion), Danny Ings (Burnley), Callum Wilson (AFC Bournemouth)
Germany
Switzerland
Hungary
Scotland
Spain
Italy
Croatia
Albania
England
Denmark
Slovenia
Serbia
Austria
France
Netherlands
Poland
Romania
Belgium
Slovakia
Ukraine
Portugal
Turkey
Georgia
Czech Republic