World number one Rory McIlroy will play his third straight Ryder Cup match in day two's morning fourballs but has lost his European partner from today, Sergio Garcia, and is joined by Ian Poulter.
Four-time Major winner McIlroy managed half a point from his two matches on day one, losing with Garcia in fourballs before scraping a half in the foursomes.
He will be joined in the final fourball match at Gleneagles by Poulter, who suffered his first Ryder Cup defeat in eight matches with local favourite Stephen Gallacher at the hands of young rookies Patrick Reed and Jordan Spieth earlier today.
In the opening Saturday match, in-form duo Justin Rose and Henrik Stenson will lead Europe out for the second day running after winning both of their matches on day one.
They will again face Bubba Watson in fourballs, but this time the Masters champion is alongside Matt Kuchar instead of Webb Simpson.
Jamie Donaldson and Lee Westwood, after victory together in the foursomes, will play fourballs together in the second match against another new American duo, Jim Furyk and Hunter Mahan.
The third match sees Reed and Spieth again together and facing Thomas Bjorn and Martin Kaymer, who are another familiar duo from the morning.
Europe's only new pairing is McIlroy and Poulter, who are up against Jimmy Walker and Rickie Fowler. The American duo played all 36 holes today and halved both of their matches.
The hosts won Friday's foursomes by three-and-a-half points to half a point for the Americans to grab a 5-3 overall lead.
Day two morning fourballs in full:
Justin Rose & Henrik Stenson vs. Bubba Watson & Matt Kuchar
Jamie Donaldson & Lee Westwood vs. Jim Furyk & Hunter Mahan
Thomas Bjorn & Martin Kaymer vs. Patrick Reed & Jordan Spieth
Rory McIlroy & Ian Poulter vs. Jimmy Walker & Rickie Fowler